. Author manuscript; available in PMC: 2011 Mar 1.

Published in final edited form as: J Pharmacol Toxicol Methods. 2010 Jan 22;61(2):136–145. doi: 10.1016/j.vascn.2010.01.001

Table 4.

ICE Irritancy Classification System

Parameter	Measurement	Score	Category^*
Corneal thickness	Mean corneal swelling	Percentage	I to IV
Corneal Opacity	Mean maximum score	0 to 4.0	I to IV
FR	Mean FR score	0 to 3.0	I to IV

Abbreviations: ICE = isolated chicken eye test method; FR = fluorescein retention.

Category assignments for test substances include: I, non-irritant; II, slight irritant; III, moderate irritant; or, IV, severe irritant.