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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2010 Jan 11;244(3):254–262. doi: 10.1016/j.taap.2009.12.038

Fig. 3. Exposure of PMKC to Cd reduces in vivo binding of Sp1 to the GC boxes in the promoters of SGLT1 and SGLT2 genes.

Fig. 3

Fig. 3

PMKC were either left untreated (M) or treated for 24 hr with 5 μM CdCl2 (Cd). ChIP assay was performed using Sp1 antibody (12 μg) (IP) or with un-immunized rabbit serum for SGLT1 and without antibody for SGLT2 as background. PCR was performed on input DNA and on chromatin DNA isolated by ChIP using primers specific to either the GC-1 site of SGLT1 (A) or to the GC site of SGLT2 (C) genes. The intensity of the PCR bands from ChIP assays were normalized to the input DNA, and then background values were subtracted from that of the chromatin immunoprecipitated with Sp1 and graphed (B,D). L, 20 bp DNA ladder. (N = 5 for SGLT1; N = 3 for SGLT2).