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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2010 Jan 11;244(3):254–262. doi: 10.1016/j.taap.2009.12.038

Fig. 4. Exposure of PMKC to Cd reduces binding of nuclear Sp1 to SGLT2-GC.

Fig. 4

EMSA was performed with DIG-labeled SGLT2-GC (0.08 pmol) and 1 μg nuclear proteins from untreated (NE-M) or 7.5 μM CdCl2 treated (NE-Cd) cultured mouse kidney cells. The intensities of the nuclear Sp1/probe bands were measured and the mean values ± SEM were graphed. (N = 2). A representative EMSA is shown above the graph.