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. Author manuscript; available in PMC: 2011 Apr 5.
Published in final edited form as: J Proteome Res. 2010 Apr 5;9(4):1786–1794. doi: 10.1021/pr900909t

Figure 5.

Figure 5

Co-immunoprecipitation of vimentin and HSL. Cell lysates (250 μg) from adipose cells were immunoprecipitated with anti-HSL IgG and then immunoblotted with anti-vimentin antibody as described in the Materials and Methods. Lane 1, control adipose lysates; lane 2, lysates of adipose cells treated with isoproterenol (1 μM); lane 3, lysates of adipose cells treated with isoproterenol (1 μM) and insulin (100 μU/ml).