Fig. 3.
NSC-127102 inhibits serine 276 phosphorylation in vitro. CREB or p65 was used as a substrate for phosphorylation of CREB at serine 133 or phosphorylation of p65 at serine 276 by MSK-1 or PKA. CREB and p65 were pre-incubated with 50 or 100 μM NSC-127102 for five minutes prior to the addition of MSK-1 or PKA. Immunoblot analysis for p-S133 of CREB and p-S276 of p65 was performed. Results are representative of three separate experiments.