Fig. 6.

The CDV fusogenic complexes are potentially functional in DBCCs. a, b Both CDV surface glycoproteins are properly expressed in glial cells. DBCCs were infected with rA75/17^red with an MOI of 0.01. Merged images of both glycoproteins F and H stained with anti-F and anti-H antibodies, respectively, followed by secondary alexa fluor 488-conjugated antibody (green) and astrocytes labeled using an anti-GFAP antibody, followed by a secondary alexa fluor 555-conjugated antibody (red). Confocal laser microscopy ×200. c, d F/H complexes are cell surface targeted in potentially functional. Prior to infection of DBCCs with rA75/17^red (MOI of 0.01), cells were transduced with a lentivirus vector expressing SLAM (pRRL-SLAM) or with a control empty plasmid (pRRL). Syncytia formation was readily observed in CDV-infected cells. No syncytia are demonstrated in the control culture