Fig. 7.

Characterization of the recombinant H^ko rA75/17^red virus. Vero-SLAM (a–f) and Vero-SLAM-H cells (g–i) were infected with rA75/17^red and rA75/17^red/H^ko/H^comp. One day post-infection, both cells were fixed, permeabilized and stained using the anti-H MAb 1C42H11 (VMRD) to detect the CDV hemagglutinin protein. This was followed by addition of an alexa fluor 488-conjugated secondary antibody. In addition, auto-fluorescence of the RFP is also shown, which allowed us for a direct illumination of infected cells. Merged images are also represented (c, f, i and l). Fluorescent images were taken using a confocal scanning laser microscope (Olympus) (×200)