Figure 4. Calcium influx is essential for glutamate-induced internalization.

Glutamate-induced internalization of Ca_v1.3 channels observed in normal Ringer solution (2 mm CaCl₂) (A), was prevented by pre-treatment of cells for 10 min with 10 μm BAPTA-AM (B), or by removal of Ca²⁺ from external solution (C). Intensity profiles are shown in insets. D, the bar graph represents normalized (to control) F_c/F_m values obtained under different experimental conditions. Data values represent mean F_c/F_m±s.e.m. (n= 20–50, P < 0.001).