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. Author manuscript; available in PMC: 2010 Apr 6.
Published in final edited form as: J Immunol. 2008 Nov 15;181(10):6797–6802. doi: 10.4049/jimmunol.181.10.6797

FIGURE 1.

FIGURE 1

Stimulation of macrophages with LPS induces the phosphorylation of GSK3-β (S9) in both wild-type and MyD88-deficient macrophages. A, Wild-type and MyD88-deficient macrophages were stimulated with 1 μg/ml LPS for 30 or 60 min. To assess phospho-GSK3-β (S9), 15 μg of total cell lysate was resolved on LDS-PAGE, immunoblotted with an anti-phospho GSK3-β (S9) Ab, and developed by ECL. Immunoblots were stripped and reprobed with an Ab to total β-actin to ensure equal protein loading. B and C, Densitometer scans of phospho-GSK3-β (S9) and total β-actin were performed and recorded as the ratio of phospho-GSK3-β (S9):total β-actin. Data are representative of three separate experiments.