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. Author manuscript; available in PMC: 2010 Apr 6.
Published in final edited form as: Structure. 2008 Dec 10;16(12):1849–1859. doi: 10.1016/j.str.2008.10.010

Table 1.

Kinetic Parameters for Wild-type E1p, S1-E1p and Phospho-S1-E1p

Assays kcat (s-1) Km for pyruvate (mM) kcat/Km (s-1mM-1)
Overall
WT E1p 21 ± 1 41 ± 4 0.51
Y89F-α E1p 19 ± 3 78 ± 13 0.24
S1-E1p 18 ± 2 58 ± 5 0.31
Phospho-S1-E1p NM NM -
DCPIP
WT E1p 0.26 ± 0.02 8.1 ± 0.5 0.032
Y89F-α E1p 2.7 ± 0.05 31 ± 5 0.087
S1-E1p 0.31 ± 0.02 9.5 ± 0.8 0.032
Phospho-S1-E1p 0.21 ± 0.01 281 ± 21 0.00075
Reductive acetylation
WT E1p 0.030 ± 0.002 - -
Y89F-α E1p 0.028 ± 0.002
S1-E1p 0.028 ± 0.001 - -
Phospho-S1-E1p NM - -

Phosphorylation studies on S1-E1p or Ser264-α (site 1) were carred out using a double alanine mutant S271A-α (site 2) and S203A-α (site 3). NM: not measurable.

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