. Author manuscript; available in PMC: 2010 Apr 6.

Published in final edited form as: Structure. 2008 Dec 10;16(12):1849–1859. doi: 10.1016/j.str.2008.10.010

Table 1.

Kinetic Parameters for Wild-type E1p, S1-E1p and Phospho-S1-E1p

Assays	k_cat (s^-1)	K_m for pyruvate (mM)	k_cat/K_m (s^-1mM^-1)
Overall
WT E1p	21 ± 1	41 ± 4	0.51
Y89F-α E1p	19 ± 3	78 ± 13	0.24
S1-E1p	18 ± 2	58 ± 5	0.31
Phospho-S1-E1p	NM	NM	-
DCPIP
WT E1p	0.26 ± 0.02	8.1 ± 0.5	0.032
Y89F-α E1p	2.7 ± 0.05	31 ± 5	0.087
S1-E1p	0.31 ± 0.02	9.5 ± 0.8	0.032
Phospho-S1-E1p	0.21 ± 0.01	281 ± 21	0.00075
Reductive acetylation
WT E1p	0.030 ± 0.002	-	-
Y89F-α E1p	0.028 ± 0.002
S1-E1p	0.028 ± 0.001	-	-
Phospho-S1-E1p	NM	-	-

Phosphorylation studies on S1-E1p or Ser264-α (site 1) were carred out using a double alanine mutant S271A-α (site 2) and S203A-α (site 3). NM: not measurable.