Figure 1.

Maize annexin preparation forms a Ca²⁺-permeable conductance in MDA-modified planar lipid bilayers. A, Schematic of experimental conditions. Highly purified maize annexin preparation (3 μg) was added to the cis-chamber. Chambers were separated by a MDA-POPE:POPC:POPS (5:3:2) bilayer formed across a hole (200 μm diameter) in the septum (black circle). Positive charge flowing from trans to cis is plotted as negative current, while that flowing from cis to trans is plotted as positive current. B, Representative current traces recorded in response to the step voltage protocol applied at −200, −100, and +100 mV from a 0-mV baseline prior to annexin addition; both chambers were at pH 6. C, Representative current traces recorded in response to voltage applied at −200, −100, and +100 mV from a 0-mV baseline, generated after addition of highly purified maize annexin (+ ANN) preparation. Currents at remaining voltages are not shown for clarity.