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. 2010 Feb 12;152(4):1824–1829. doi: 10.1104/pp.109.145458

Figure 2.

Figure 2.

Rectification, effect of pH, and pharmacology. A, Comparison of IV relationships generated by 3 μg of annexin (ANN) preparation (added to the cis-chamber) at different pH values across the MDA-POPE bilayer. In these experiments, the Ca2+ gradient was the same as in Figure 1. For pH 4, only values from −200 to 0 mV were determined. Data are mean ± se (n = 5 for pH 6; n = 3 for pH 4; n = 5 for heat-inactivated preparation at pH 6). With pH 4 across the MDA bilayer, mean current evoked at −200 mV was −23 ± 3 pA, a 32% decrease compared with pH 6 but not significantly different. B, IV relationships generated by 3 μg of annexin preparation (cis-chamber), MDA-POPE bilayer with a Ca2+ gradient of cis 1 μm and trans 5 mm, pH 6 (n = 3). Predicted ECa was +103 mV; ECl was −215 mV. No clear Erev was seen due to rectification. Mean current evoked at −200 mV was −55 ± 9 pA. Addition of 50 μm Gd3+ to the trans-chamber abolished current (n = 3).