Figure 3.
Overexpression of miR160a enhances PAMP-induced callose deposition. A, Accumulation of miR160a in 35S:miR160a plants. Twenty micrograms small RNA was loaded for RNA-blot analysis. B, Overexpression of miR160a down-regulates ARF16 and ARF17 mRNA in transgenic plants. RNA was extracted from T2 generation of 35S:miR160a plants for quantitative real-time RT-PCR analyses. Error bars indicate sd. C, Flg22- and hrcC−-induced callose deposition in 35S:miR160a plants. D, 35S:miR160a plants were not affected in resistance to DC3000 bacteria. Wild-type (WT) and 35S:miR160a transgenic plants were infiltrated with 5 × 105 cfu/mL DC3000 bacteria, and leaf bacterial population was determined at the indicated times. Error bars indicate sd. The experiment was repeated three times with similar results. Student's t test was carried out to determine the significance of difference between 35S:miR160a and wild-type plants within each treatment. Asterisks (* and **) indicate significant difference at a P value of <0.05 and <0.01, respectively.