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Polyphosphoinositides are enriched in DIMs. A, Specificity of the antibody to PtdIns(4,5)P2 tested by dot blot and immunodetection. Each lipid solution was dried, and lipids were resuspended in 50 μL of TBS-T, sonicated for 5 min, and plotted on a PVDF membrane. GluCER, Glucosylceramide. B, PtdIns(4,5)P2 was enriched in DIMs. Five or 10 μg of PM or DIMs purified from BY-2 cells was analyzed by dot blot and immunodetection using an antibody to PtdIns(4,5)P2.
