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. 2010 Feb 26;152(4):2258–2268. doi: 10.1104/pp.109.150979

Figure 6.

Figure 6.

Effect of amiFLS in AtMYB12-expressing transgenic lines. A and B, Flower color phenotype (A) and decrease in rutin content by amiFLS (B) in AtMYB12-expressing transgenic lines. FW, Fresh weight. C, Insect bioassay in the wild type (WT), AtMYB12-expressing (TAtMyb12), and AtMYB12-expressing but NtFLS-suppressed (TAtMyb12:MiFLS) transgenic line 7. Two-day-old S. litura larvae were released on detached leaves of the plants followed by observations after 3 d. Decreased rutin content in AtMYB12-expressing transgenic lines through amiFLS shows reversion of insect resistance, suggesting the involvement of rutin in insect resistance. D and E, RT-PCR analysis and relative expression of NtFLS in the wild type, AtMYB12-expressing, and amiFLS-suppressed transgenic line 7. Expression of NtFLS decreased due to the expression of amiFLS in the AtMYB12-expressing transgenic lines. Expression of ubiquitin was used for normalization. [See online article for color version of this figure.]