Figure 2.

Map-based cloning of SIS3. A, Map of chromosome 3 surrounding SIS3. The number of recombinants (rec) found in 1,200 DNA samples is shown. SIS3 is located between 17,701 and 17,774 kb on bacterial artificial chromosome (BAC) T17F15. B, Structure of the SIS3 genomic sequence displaying positions of T-DNA insertions relative to the beginning of the start codon. Boxes represent exons and lines represent introns. Black boxes denote coding regions. C, Quantitative real-time RT-PCR analysis of relative SIS3 transcript levels in mutant and wild-type (Col-0) plants. Binding locations of primer pairs A and B are denoted by the short arrows in B. Error bars indicate sd.