Figure 1.

Overexpression of Arabidopsis NADK2 in transgenic rice plants. A, Construction of the chimeric gene used for generation of Arabidopsis NADK2-overexpressing transgenic plants. The coding sequence of Arabidopsis NADK gene (AtNADK2) was cloned between the maize ubiquitin promoter and nos terminator. B, RT-PCR analysis of the AtNADK2 transgene expression. Total RNA was prepared from the vector control (C) or the NK2 lines. PCR primers specific to the NADK2 transgene or β-tubulin were used. C, Total NADK activity in leaf samples from three NK2 rice lines and vector control. Samples were taken from 1-month-old plants. Data are mean ± sem of measurements of five individual plants per line. *P < 0.05, compared with the vector control (by Student's t test).