Figure 4.

Virus-induced gene silencing of NbSGLP and effect of silencing of NbSGLP on the expression of defense-related genes. N. benthamiana plants were infected with A. tumefaciens carrying either PVX (gray bars) or PVX:SGLP (black bars). Three weeks later, the fourth leaves above the primary A. tumefaciens-infected leaves were infiltrated with Rs8107, and total RNA was isolated at the indicated time points. The relative abundances of NbSGLP, PR-1a, PR-4, and EREBP transcripts were analyzed using quantitative real-time PCR with primer combinations described in Supplemental Table S1. Quantitative real-time PCR was carried out as described in “Materials and Methods.” Expression values of NbSGLP are expressed as Qty values after normalization with the actin value. Expression values of PR-1a, PR-4, and EREBP are relative to the absolute nontreated control level and are normalized against the actin values. Values represent means and sd of results from triplicate experiments. Asterisks denote values significantly different from empty PVX controls (P < 0.05).