Figure 5.

Effects of NbSGLP silencing on growth of R. solanacearum and development of bacterial wilt. N. benthamiana leaves were preinoculated with A. tumefaciens either carrying empty PVX (gray bars) or PVX:SGLP (black bars). A, Control and NbSGLP-silenced leaves were infiltrated with a bacterial suspension of Rs8107. B, Three weeks later, the fourth leaves above the primary A. tumefaciens-infected leaves were infiltrated with a bacterial suspension of RsOE1-1. The bacterial population was determined by plating at specified time points. Values are means of four replicate experiments with sd. Asterisks denote values significantly different from empty PVX controls (P < 0.05). C, Disease development of bacterial wilt was rated daily on a 0 to 4 disease index in empty PVX (white circles) or PVX:SGLP (black boxes). Each point represents the mean disease index of 10 plants combined from three separate experiments. Asterisks denote values significantly different from empty PVX controls (P < 0.05). D, Characteristic symptoms of bacterial wilt in control (PVX) and NbSGLP-silenced (PVX:SGLP) N. benthamiana. The photograph was taken 12 d after inoculation with RsOE1-1. [See online article for color version of this figure.]