Figure 2.

Fluorescent probes for transport vesicles, endosomes, and the actin cytoskeleton expressed in transgenic Arabidopsis pollen tubes. A, A pollen tube expressing YFP-RabA4b. B, A pollen tube expressing ARA6-YFP. C, A pollen tube expressing YFP-ARA7. D, A pollen tube expressing YFP-mTalin. Arrows in B and C indicate the apical clear zone, in which ARA6-labeled endosomes are excluded while ARA7-labeled endosomes are present. Asterisk in D marks the apical F-actin ring. Scale bar = 20 μm. [See online article for color version of this figure.]