Table I. Nano LC-ESI-MS/MS analysis of proteins binding to the E-box region.
Protein Identifier |
Name/Conserved Domains | Identified Peptides | z | Xcorr | |
Chlre2.0 | Chlre4.0 | ||||
154362 | 146567 | G-strand telomere-binding protein 1; CDs: 2× RRM; Expect = 1e-19, 3e-14 | DMFAEVGGVDR | 2 | 3.18 |
QADGAPGAPAER | 2 | 2.93 | |||
RLAVFIDR | 2 | 3.03 | |||
ADVVTGYDGR | 2 | 3.17 | |||
158312 | 129012 | High-light-induced nuclease; CDs: staphylococcal nuclease homolog domain; Expect = 8e-21; InterPro: transcription factor TFIIB-related domain | FFCTESEATSAGWR | 2 | 3.96 |
GIWAGEFQVPSEWR | 2 | 4.15 | |||
164634 | 137062 | Predicted protein; CD: short-chain dehydrogenase/reductase; Expect = 9e-46 | GLAAELGPEGIRa | 2 | 3.29 |
152972 | 59969 | Predicted proteinb; CD: short-chain dehydrogenase/reductase; Expect = 2e-64 | AEGVTAMGLQGDVR | 2 | 4.26 |
ELVQSTIPLR | 2 | 2.71 | |||
SAVDSLTR | 1 | 2.08 | |||
SLALEWGEFNVR | 2 | 4.17 | |||
VALVTGGSSGIGFEIAR | 2 | 3.71 | |||
VNGVAPGPIDGTAGMTK | 2 | 3.06 | |||
162329 | 307591 | Predicted proteinb; CD: HU_IHF, a member of the DNABII protein family that binds and bends DNA, functioning as architectural factors in many cellular processes, including transcription, site-specific recombination, and higher order nucleoprotein complex assembly; Expect = 2e-23 | AFDSLIGGIEDALINGDR | 2 | 2.65 |
LVEAIATEVGLTK | 2 | 4.59 | |||
NPSTGAVLQIAASK | 2 | 4.01 |
The spectra of the peptide were manually evaluated.
The protein was only identified in one of two independent large-scale experiments.