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. 2010 Feb 12;152(4):2243–2257. doi: 10.1104/pp.109.149195

Table I. Nano LC-ESI-MS/MS analysis of proteins binding to the E-box region.

The name/conserved domains (CD) of depicted proteins are given as determined by National Center for Biotechnology Information BLAST and their e-values. Xcorr, Cross-correlation factor; z, charge.

Protein Identifier
Name/Conserved Domains Identified Peptides z Xcorr
Chlre2.0 Chlre4.0
154362 146567 G-strand telomere-binding protein 1; CDs: 2× RRM; Expect = 1e-19, 3e-14 DMFAEVGGVDR 2 3.18
QADGAPGAPAER 2 2.93
RLAVFIDR 2 3.03
ADVVTGYDGR 2 3.17
158312 129012 High-light-induced nuclease; CDs: staphylococcal nuclease homolog domain; Expect = 8e-21; InterPro: transcription factor TFIIB-related domain FFCTESEATSAGWR 2 3.96
GIWAGEFQVPSEWR 2 4.15
164634 137062 Predicted protein; CD: short-chain dehydrogenase/reductase; Expect = 9e-46 GLAAELGPEGIRa 2 3.29
152972 59969 Predicted proteinb; CD: short-chain dehydrogenase/reductase; Expect = 2e-64 AEGVTAMGLQGDVR 2 4.26
ELVQSTIPLR 2 2.71
SAVDSLTR 1 2.08
SLALEWGEFNVR 2 4.17
VALVTGGSSGIGFEIAR 2 3.71
VNGVAPGPIDGTAGMTK 2 3.06
162329 307591 Predicted proteinb; CD: HU_IHF, a member of the DNABII protein family that binds and bends DNA, functioning as architectural factors in many cellular processes, including transcription, site-specific recombination, and higher order nucleoprotein complex assembly; Expect = 2e-23 AFDSLIGGIEDALINGDR 2 2.65
LVEAIATEVGLTK 2 4.59
NPSTGAVLQIAASK 2 4.01
a

The spectra of the peptide were manually evaluated.

b

The protein was only identified in one of two independent large-scale experiments.