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. Author manuscript; available in PMC: 2010 Nov 25.
Published in final edited form as: Neuron. 2009 Nov 25;64(4):484–497. doi: 10.1016/j.neuron.2009.09.027

Figure 2. Dscam is required for retinal ganglion cell spatial patterning but does not confer cell type identity.

Figure 2

Retinal ganglion cell populations were labeled in adult wild type and Dscam−/− retinas using the antibody SMI-32 to non-phosphorylated neurofilament (A and D; alpha RGCs), anti-melanopsin (B and E; melanopsin-positive RGCs), and the Mito-Y transgene (C and F; Mito-Y positive RGCs). A and D, Alpha RGCs are aggregated in the periphery of the Dscam−/− retina compared to the wild type retina, arrowheads denote cell bodies in the central retina of wild type mice. B and E, Melanopsin-positive RGCs are densely aggregated in the Dscam−/− retina compared to wild type. The dendrites of melanopsin-positive RGCs form fascicles in the Dscam−/− retina, whereas they arborize in wild type. C and F, Mito-Y positive RGCs are aggregated and fasciculated in the Dscam−/− retina. G–L, Pair-wise labeling of adult wild type and Dscam−/− retinas with antibodies to melanopsin, non-phosphorylated neurofilament (SMI-32), and the Mito-Y transgene (N>3). RGC aggregates and fascicles in the Dscam−/− retina were composed primarily of a single RGC type (H, J and L). M, RGC cell types form mosaics in the wild type retina and aggregate and fasciculate with cells of the same type in the Dscam−/− retina. The scale bar in (H) is equivalent to 280 µm in A-–F and 387.5 µm in G–L.