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. Author manuscript; available in PMC: 2010 Apr 6.
Published in final edited form as: Cell. 2000 Aug 4;102(3):377–385. doi: 10.1016/s0092-8674(00)00042-8

Figure 2. Affinity Purified Espin Antibody Labels Hair Cell Stereocilia in Whole Mounts of Isolated Cochlear Sensory Epithelium from a 13-day Chicken Embryo (Confocal Immunofluorescence Method).

Figure 2

(A) Low magnification view from above the epithelium. Bar in (C), 28 μm.

(B) Higher magnification view showing several clumps of labeled stereocilia emanating from the apex of a single hair cell. Bar in (C), 5 μm.

(C) Double labeling of the same hair cell shown in (B) with rhodamine-phalloidin reveals the intensely labeled bundles of F-actin at the cores of the clumped stereocilia emanating from a less intensely labeled cuticular plate. Bar, 5 μm.