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. Author manuscript; available in PMC: 2010 Apr 6.
Published in final edited form as: Cell. 2000 Aug 4;102(3):377–385. doi: 10.1016/s0092-8674(00)00042-8

Figure 4. Deficiency of Espin Protein, but Presence of Espin mRNA, in the Testis and Kidney of Adult Jerker Homozygotes.

Figure 4

(A-C) Western blots of homogenates prepared from testes or kidneys of duplicate CBA/CaJ wild-type mice (CBA), jerker heterozygotes (+/je), or jerker homozygotes (je/je) labeled with affinity purified espin antibody. Note the absence of specific labeling in the molecular mass regions expected for espin [~110 kD (A and B), arrowheads] or small espin [~30 kD (C), arrowhead] in jerker homozygotes. In (A) and (C), the affinity purifed espin antibody was directed against the 167-residue shared C-terminal peptide of the rat espins [ESPIN(671-837)]. In (B), the affinity purified espin antibody was directed against a 262-residue peptide from a more central region of rat espin [ESPIN(459-720)]. Bracket in B, ~70-kD doublet that shows weak cross-reaction with the antibody directed against the more central region of espin, but is also present in the samples from the CBA wild-type mice.

(D and E) RT-PCR analysis of total RNA isolated from the testis (D) or kidney of (E) of two jerker heterozygotes (+/je) and two jerker homozygotes (je/je) using primers that amplify a 288-bp fragment from the 3′ end of the coding region of the espins showing cDNA products of roughly comparable levels and sizes.