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. Author manuscript; available in PMC: 2010 Apr 6.
Published in final edited form as: J Immunol. 2009 Jan 1;182(1):547–553. doi: 10.4049/jimmunol.182.1.547

FIGURE 3.

FIGURE 3

Ability of GSK3 to regulate phospho-ERK levels in monocytes is dependent upon Rac1. A, Immunoblots showing GSK3-inhibition (LiCl at 10 mM) does not affect the levels of phospho- c-Raf (S259 or S621) in LPS-stimulated (1 µg/ml) cells. B, Immunoblot demonstrating LiCl (10 mM) mediated GSK3-inhibition suppressed the levels of phospho-Rac1 (S71) in LPS-stimulated (1 µg/ml) monocytes. C, siRNA-mediated knockdown of Rac1 inhibits the ability of GSK3 inhibition (LiCl at 10 mM) to increase phospho-ERK levels in LPS-stimulated (1 µg/ml) monocytes. siRNA-mediated knockdown of Rac1 levels was determined by Western blot 72 h post transfection. A–C, Immunoblots were reprobed for total p38 or total ERK to ensure equivalent protein loading. Data are representative of three experiments.