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. Author manuscript; available in PMC: 2010 Apr 6.
Published in final edited form as: J Immunol. 2009 Jan 1;182(1):547–553. doi: 10.4049/jimmunol.182.1.547

FIGURE 6.

FIGURE 6

Model of how GSK3 regulates IL-1Ra production by LPS-stimulated monocytes. Inhibition of GSK3 suppressed phospho-Rac1 (S71) levels and augmented the ability of active Rac1 to associate with PAK. siRNA-mediated knockdown in Rac1 levels attenuated the ability of GSK3 inhibition to increase phospho-ERK levels in LPS-stimulated cells. GSK3 inhibition increased phospho-c-Raf levels, in which c-Raf inhibition blocked the increased levels of phospho-MEK and ERK1/2. Inhibition of MEK1/2 demonstrated that the ability of GSK3 to augment IL-1Ra production was dependent upon ERK1/2 activity.