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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Jan 15;1803(2):323–332. doi: 10.1016/j.bbamcr.2010.01.006

Figure 4. IKKα suppresses UVB-induced cyclin d1 transcription.

Figure 4

(A) A Cyclin D1 promoter driven-luciferase reporter plasmid was transfected into WT and IKKα-null cells and stable transfectants were established. The cells were then exposed to different doses of UVB and the luciferase activity was measured 12 hrs after stimulation. (B) WT and IKKα-null cells were exposed to different doses of UVB and RT-PCR assay was performed 12 hrs after stimulation to detect cyclin d1 transcripts. The relative expression levels of cyclin d1 mRNA normalized to the β-actin control are presented. (C) WT, IKKα−/− and IKKα−/− (IKKα) cells stably transfected with the Cyclin D1-luciferase reporter plasmid were exposed to different doses of UVB and their luciferase activities were measured 12 hrs after stimulation.