Fig. 10.
Changes in expression of genes that regulate epithelial-to-mesenchymal transitions (EMT) and Hh pathway activation during CCl4-induced cirrhosis. Liver RNA was isolated from mice that had been treated with CCl4 for 8 wk to induce cirrhosis and compared with age- and sex-matched littermates treated with corn oil vehicle (n = 6 each). qRT-PCR was used to assess expression of Hh signaling factors known to regulate EMT. A: Shh, Gli2, and Hh-interacting protein (Hhip). B: epithelial genes that inhibit EMT (desmoplakin, BMP-7, and Id2). C: factors that promote EMT (TGF-β and S100A4). *P < 0.05.