Fig. 4.

Culture-induced changes in protein expression of EMT-related factors during transition of Q-HSC into MF-HSC. A: Oil Red O staining to detect neutral lipids and immunocytochemistry for K19, an epithelial keratin, in a representative, freshly isolated HSC from preparations described in Fig. 1. Immunocytochemistry in NRC confirms K19 positivity. Magnification ×40. B: Western blot analysis of protein harvested from these primary rat HSC. Results are representative of triplicate experiments.