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Effects of inhibiting Hh signaling on protein expression of representative epithelial and mesenchymal markers in cultured primary rat HSC. Whole cell protein was isolated from HSC cultures described in Fig. 5. Changes in protein expression of K7, K19, α-SMA, and BMP-7 were assessed by Western blot analysis (40 μg whole cell protein/lane). β-Actin was used as a loading control.
