Fig. 8.

Effects of Hh pathway inhibition on expression of EMT-related genes in primary human MF-HSC. Primary human HSC described in Fig. 7 were treated with tomatidine or cyclopamine, and gene expression changes were compared by qRT-PCR. A: Hh signaling pathway factors (Gli1 and Gli2) and EMT inhibitors (BMP-7 and Id2). B: EMT promoters (Snail, Slug, and S100A4). C: epithelial markers (E-cadherin and desmoplakin) and the HSC quiescence marker PPARγ. D: myofibroblastic markers [α-SMA, Col1α₁, and plasminogen activator inhibitor-1 (PAI-1)]. Values are means ± SE of triplicate experiments. *P < 0.05; **P < 0.01.