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Effects of Hh signaling inhibition on protein expression of representative epithelial and mesenchymal markers in cultured primary human HSC. Whole cell protein was isolated from HSC cultures described in Fig. 7. Changes in protein expression of K7, K19, α-SMA, Gli2, BMP-7, and Id2 were assessed by Western blot (40 μg whole cell protein/lane). β-Actin was used as a loading control.
