Table 1.
RT-PCR primers for analysis
| Gene | GenBank Accession No. | Sequence |
|---|---|---|
| α−SMA | X06801 | |
| Forward | GTGGATCACCAAGCAGGAGGAGT | |
| Reverse | CATAGCACGATGGTCGATTG | |
| Col1α1 | XM_213440 | |
| Forward | CTGCATACACAATGGCCTAA | |
| Reverse | GGGTCCCTCGACTCCTA | |
| Fibronectin | NM_019143 | |
| Forward | GTGGCTGCCTTCAACTTCTC | |
| Reverse | GTGGGTTGCAAACCTTCAAT | |
| TGF-β | NM_021578 | |
| Forward | TTGCCCTCTACAACCAACACAA | |
| Reverse | GGCTTGCGACCCACGTAGTA | |
| PPARγ | NM_013124 | |
| Forward | CCCTGGCAAAGCATTTGTAT | |
| Reverse | ACTGGCACCCTTGAAAAATG | |
| GFAP | NM_017009 | |
| Forward | GGGAGTCGGCCAGTTACCAG | |
| Reverse | CCCGCATCTCCACCGTCT | |
| K7 | NM_033073 | |
| Forward | TAGAGTCCAGCATCGCAGAG | |
| Reverse | CACAGGTCCCATTCCGTC | |
| K19 | NM_199498 | |
| Forward | GTCCACACTACGCAGATCCA | |
| Reverse | CAAGCAGGCTTCGGTAGGT | |
| S100A4 | NM_012618 | |
| Forward | ATACTCAGGCAACGAGGGTG | |
| Reverse | CTTCCGGGGCTCCTTATC | |
| BMP-7 | XM_342591 | |
| Forward | GTGGTCAACCCTCGGCACA | |
| Reverse | GGCGTCTTGGAGCGATTCTG | |
| Desmoplakin | XM_001058477 | |
| Forward | GGAAGTCAGCCAAGCAAAAC | |
| Reverse | GGCTCTCCTTTTCACACTGC | |
| E-cadherin | NM_009864 | |
| Forward | ACCTCTGGGCTGGACCGA | |
| Reverse | CCTGATACGTGCTTGGGTTGAA | |
| Shh | NM_017221 | |
| Forward | ACAAGAAACTCCGAACGATT | |
| Reverse | GCCCTCAGTCACTCGAAG | |
| Hhip | XM_238042 | |
| Forward | TGTGCCGTGGATCGAC | |
| Reverse | GATCTCCGAACACGTAGCTT | |
| Gli2 | XM_222557 | |
| Forward | ATAAGCGGAGCAAGGTCAAG | |
| Reverse | CAGTGGCAGTTGGTCTCGTA | |
| S9 | NM_029767 | |
| Forward | GACTCCGGAACAAACGTGAGGT | |
| Reverse | CTTCATCTTGCCCTCGTCCA | |
| Col1α1 | NM_000088 | |
| Forward | TGTGAGGCCACGCATGAG | |
| Reverse | CAGATCACGTCATCGCACAA | |
| PAI-1 | NM_000602 | |
| Forward | CTCTCTCTGCCCTCACCAAC | |
| Reverse | GTGGAGAGGCTCTTGGTCTG | |
| PPARγ | NM_138712 | |
| Forward | CGTGGCCGCAGATTTGAA | |
| Reverse | CTTCCATTACGGAGAGATCCAC | |
| β-Actin | NM_001101 | |
| Forward | TGGCATCCACGAAACTACCT | |
| Reverse | ACGGAGTACTTGCGCTCAG | |
| Snail | NM_053805 | |
| Forward | CATCCCTCCTGCGTCC | |
| Reverse | CCTAACAAGTGACGGCCATT |
α-SMA, α-smooth muscle actin; Col1α1, collagenase 1α1; TGF-β, transforming growth factor-β; PPARγ, peroxisome proliferator -activated receptor-γ; GFAP, glial fibrillary acidic protein; K7 and K19, ketatins 7 and 19; BMP-7, bone morphogenic protein-7; Shh, Sonic Hedgehog; Hhip, Hedgehog-interacting protein; PAI-1, plasminogen activator inhibitor-1.