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Time- and concentration-dependent effect of taurolithocholate (TLC) on taurocholate (TC) uptake. HuH7 cells stably transfected with human Na+-dependent TC-cotransporting polypeptide (NTCP) (HuH-NTCP cells) were incubated with 10 μM TLC for indicated time (A) or with indicated TLC concentrations for 25 min (B) before determining TC uptake. Values are means ± SE (n = 4). *Significantly different (P < 0.05) from 0 min (A) or 0 μM TLC (B) values.
