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Effect of DN-PKCε on PKB phosphorylation by TLC and PMA. HuH-NTCP cells were transfected with 1 μg DN-PKCε or empty vector followed by incubation with 10 μM TLC or 1 μM PMA for 15 min. Cell lysates were subjected to immunoblot analysis for HA with actin for loading control, pPKB, and tPKB. Top: typical immunoblot analysis. Bottom: result of densitometric analysis (means ± SE; n = 3). *Significantly different from control EV value (P < 0.05).
