Fig. 3.
Competitive inhibition of TC uptake by TLC. HuH-NTCP cells were incubated with 0, 2.5, 5.0, or 10.0 μM TLC for 15 min followed by determination of TC (5.0, 10.0, 20.0, and 50.0 μM) uptake. Concentration-dependent effect of TLC on TC uptake (means ± SE, n = 3) is shown in A, and Dixon and Cornish-Bowden plots of data in A showing competitive inhibition are presented in B and C, respectively. The solid lines represent the regression lines through the data points. The estimated Km value for TC and the estimated inhibitor constant (Ki) for TLC were 7.9 ± 0.13 μM and 1.1 ± 0.03 μM, respectively.