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Phosphoinositide-3-kinase (PI3K)-independent inhibition of TC uptake by TLC. HuH-Ntcp cells were pretreated with or without 20 μM LY294002 for 20 min and then with different concentrations of TLC or 100 μM CPT-cAMP before determining TC uptake. Values are means ± SE (n = 3); *P < 0.05 compared with 0 μM TLC and #P < 0.05 compared with cAMP value in the absence of LY294002.
