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Inhibition of cAMP-induced activation of PKB by LY294002 (LY). HuH-NTCP cells were pretreated with or without 20 μM LY294002 for 20 min followed by incubation with 100 μM CPT-cAMP for 15 min. Cells were also treated with 10 μM TLC for 25 min (negative control). Top: typical immunoblot analysis of pPKB and tPKB. Bottom: result of densitometric analysis (means ± SE; n = 3). *Significantly (P < 0.05) different from control values in the absence of LY294002.
