Fig. 9.

Northern blot analyses of selected mature miRNAs. Northern blotting was performed as described in materials and methods with probes for let-7a (A), miR-21 (B), or miR-451 (C). D: ethidium bromide-stained gel before transfer, showing approximately equal amounts of 5S rRNA in each lane. Lanes 1, 4, 7, and 10, RNAs isolated from free polysome fractions; lanes 2, 5, 8, and 11, cytoskeleton-associated polysome fractions; lanes 3, 6, 9, and 12, membrane-associated polysome fractions. Source of polysome population and time after PH are indicated at top and at bottom, respectively. miRNAs identified by Northern blot or the 5S rRNA are indicated at right.