Table 4.
Time After PH, h |
||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
0 |
3 |
6 |
24 |
|||||||||
miRNA | F | C | M | F | C | M | F | C | M | F | C | M |
hsa-miR-106a | 100 | 46 | 114 | 100 | 77 | 77 | 100 | 87 | 69 | 100 | 79 | 64 |
hsa-miR-130a|mmu-miR-130a|rno-miR-130a | 100 | 57 | 72 | 100 | 60 | 69 | 100 | 60 | 86 | 100 | 112 | 100 |
hsa-miR-185|mmu-miR-185|rno-miR-185 | 100 | 70 | 71 | 100 | 109 | 102 | 100 | 155 | 146 | 100 | 67 | 65 |
hsa-miR-198 | 100 | 90 | 100 | 100 | 113 | 94 | 100 | 72 | 76 | 100 | 100 | 93 |
hsa-miR-19b|mmu-miR-19b|rno-miR-19b | 100 | 60 | 57 | 100 | 75 | 61 | 100 | 38 | 58 | 100 | 40 | 52 |
hsa-miR-202 | 100 | 63 | 84 | 100 | 95 | 84 | 100 | 83 | 99 | 100 | 114 | 133 |
hsa-miR-205|mmu-miR-205|rno-miR-205 | 100 | 77 | 77 | 100 | 108 | 104 | 100 | 154 | 173 | 100 | 58 | 90 |
hsa-miR-20a|mmu-miR-20a|rno-miR-20a | 100 | 40 | 63 | 100 | 49 | 65 | 100 | 83 | 66 | 100 | 110 | 75 |
hsa-miR-30d|mmu-miR-30d|rno-miR-30d | 100 | 55 | 50 | 100 | 55 | 65 | 100 | 52 | 72 | 100 | 57 | 67 |
hsa-miR-320|mmu-miR-320|rno-miR-320 | 100 | 77 | 121 | 100 | 56 | 69 | 100 | 94 | 89 | 100 | 118 | 111 |
hsa-miR-335|mmu-miR-335|rno-miR-335 | 100 | 59 | 58 | 100 | 95 | 96 | 100 | 53 | 55 | 100 | 82 | 104 |
hsa-miR-373* | 100 | 81 | 51 | 100 | 82 | 57 | 100 | 72 | 53 | 100 | 84 | 51 |
hsa-miR-451 | 100 | 25 | 10 | 100 | 24 | 19 | 100 | 24 | 31 | 100 | 19 | 14 |
hsa-miR-494 | 100 | 85 | 101 | 100 | 53 | 71 | 100 | 73 | 85 | 100 | 82 | 83 |
hsa-miR-638 | 100 | 119 | 93 | 100 | 128 | 87 | 100 | 56 | 59 | 100 | 81 | 66 |
hsa-miR-663 | 100 | 119 | 111 | 100 | 207 | 101 | 100 | 59 | 60 | 100 | 112 | 125 |
mmu-miR-202 | 100 | 72 | 117 | 100 | 90 | 78 | 100 | 83 | 86 | 100 | 96 | 104 |
mmu-miR-20b | 100 | 39 | 44 | 100 | 41 | 80 | 100 | 81 | 69 | 100 | 91 | 79 |
mmu-miR-290|rno-miR-290 | 100 | 74 | 64 | 100 | 66 | 83 | 100 | 76 | 35 | 100 | 105 | 65 |
mmu-miR-292-5p|rno-miR-292-5p | 100 | 64 | 44 | 100 | 55 | 42 | 100 | 47 | 35 | 100 | 57 | 52 |
mmu-miR-451|rno-miR-451 | 100 | 28 | 12 | 100 | 23 | 21 | 100 | 27 | 33 | 100 | 16 | 14 |
mmu-miR-494|rno-miR-494 | 100 | 84 | 125 | 100 | 55 | 74 | 100 | 68 | 69 | 100 | 101 | 106 |
mmu-miR-667 | 100 | 101 | 64 | 100 | 103 | 71 | 100 | 89 | 65 | 100 | 121 | 63 |
mmu-miR-706 | 100 | 51 | 51 | 100 | 65 | 65 | 100 | 73 | 78 | 100 | 104 | 86 |
mmu-miR-709 | 100 | 81 | 83 | 100 | 128 | 115 | 100 | 126 | 93 | 100 | 96 | 98 |
mmu-miR-721 | 100 | 88 | 67 | 100 | 95 | 60 | 100 | 60 | 56 | 100 | 105 | 56 |
mmu-miR-744 | 100 | 124 | 122 | 100 | 219 | 125 | 100 | 37 | 51 | 100 | 69 | 83 |
mmu-miR-762 | 100 | 104 | 109 | 100 | 150 | 83 | 100 | 53 | 44 | 100 | 79 | 74 |
mmu-miR-763 | 100 | 116 | 103 | 100 | 109 | 84 | 100 | 96 | 87 | 100 | 102 | 95 |
rno-miR-140* | 100 | 83 | 76 | 100 | 85 | 76 | 100 | 81 | 81 | 100 | 92 | 78 |
rno-miR-327 | 100 | 109 | 103 | 100 | 106 | 73 | 100 | 100 | 65 | 100 | 100 | 75 |
rno-miR-422b | 100 | 53 | 60 | 100 | 78 | 86 | 100 | 80 | 97 | 100 | 67 | 65 |
Values are averages of 3 sets of biological replicates for each polysome population and time point. miRNAs are from the 85 probes that gave the highest hybridization signals on the microarray heat map shown in Fig. 7. Names of miRNAs are from the Invitrogen probe set, but only hsa (human), mmu (mouse), and rno (rat) miRNAs are listed. Polysomes were isolated from regenerating liver at indicated times after PH. F, free polysome-associated miRNAs (level of miRNA probe hybridized in this population was assigned a value of 100 at each time point); C, cytoskeleton-bound polysome miRNAs as % of free polysome miRNAs at the same times after PH; M, membrane-bound polysome miRNAs as % of free polysome miRNA levels at the same times after PH.