Figure 4.

Immunolocalization of type-I collagen (panels a and c, green) and cellular nuclei (panels b and d, blue) in the static culture (panels a and b) and in the ultrasonic culture (panels c and d), bars equal to 80 μm. During the culture period, in the control (panels a and b), the osteoblasts built a scanty amount of bone matrix, whereas, in the stimulated culture (panels c and d), the osteoblasts secreted a wide amount of matrix. The immunolocalization of osteocalcin, osteopontin, and type-III collagen revealed similar results.