Figure 4. Distribution of hexosaminidase D transcripts in murine tissues.
RT-PCR reactions were performed using cDNAs from various murine tissues which were normalised based on their glyceraldehyde-3-phosphate dehydrogenase (GAP-DH) transcript levels (lower panel); primer sets were used to either detect a small internal fragment (upper panel; ca, 150 bp) or the full length transcript (middle panel; ca. 1600 bp) of murine hexosaminidase D. The data show ubiquitous expression in all tissues examined: tibialis anterior (TA), soleus (Sol), atrium, left ventricle (Ventr), uterus, lung, liver, kidney and brain cortex.