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. Author manuscript; available in PMC: 2010 Apr 6.
Published in final edited form as: J Biol Chem. 2006 Dec 28;282(9):5984–5990. doi: 10.1074/jbc.M608087200

Figure 2. Assay of recombinant human xylosyltransferases by RP-HPLC.

Figure 2

The syndecan peptide substrate was incubated for 90 minutes with culture supernatants of pgsA-745 cells transiently transfected with plasmids with either human XT-I (A and B) or human XT-II (C and D) in the presence (A and C) or absence (B or D) of UDP-Xyl. The peaks eluting at ~10 and ~11 minutes were verified by MALDI-TOF MS to contain the xylosylated product and the substrate respectively.