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. Author manuscript; available in PMC: 2010 Apr 6.
Published in final edited form as: FEBS J. 2006 Dec 20;274(3):714–726. doi: 10.1111/j.1742-4658.2006.05615.x

Figure 7. Fucosyltransferase activities in an Ascaris suum extract.

Figure 7

Nematode extract was incubated with dabsyl-N-glycopeptides (A) MM, (B) GnGn, (C) GalGal or (D) EGNEGN (nomenclature based on that of Schachter) in the presence of GDP-Fuc for 5 hours (controls without GDP-Fuc were also performed, data not shown). The MM glycopeptide was apparently not modified, the GnGn substrate is the acceptor for a single fucose residue, the GalGal and βGNβGN for two fucose residues. Laser-induced degradation results, in part, in a decrease of m/z 132 (peaks marked by an asterisk). Hexosaminidase digestion products are indicated with −1HexNAc or −2HexNAc. Structures of substrates and products shown in the diagrammatic form of the Consortium for Functional Glycomics with black squares indicating GlcNAc, grey circles mannose, white squares GalNAc, white circles galactose and grey triangles fucose.