Figure 7. MALDI-TOF MS analysis of N-glycans from N2 wild-type and hex-2 mutant worms.
Pyridylaminated PNGase A-released N-glycans from N2 or tm2350 strains were pooled after RP-HPLC and subject to monoisotopic MALDI-TOF MS. The spectra are annotated with the m/z values of selected species and the major putative structures (as deduced by the HPLC, exoglycosidase digestion and MS-MS data shown in Figs. 8 and 9) are depicted using the nomenclature of the Consortium for Functional Glycomics. A shift to structures containing an extra HexNAc is apparent in the hex-2 mutant; the relative peak intensities for these data are summarised in Table 1.