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. Author manuscript; available in PMC: 2011 Apr 1.

Published in final edited form as: JALA Charlottesv Va. 2010 Apr 1;15(2):120–135. doi: 10.1016/j.jala.2009.10.009

a). Fluorescent intensity images gathered with a confocal laser scanner for ~100 μm diameter spots of anti-TNFa capture probe after exposure to a 1 pg/ml solution of TNFα and subsequent labeling with Cy5-labeled secondary antibody. At this concentration, detection is near the detection limit when the assay is performed on a glass surface, but the PC surface shows robust signal-to-noise ratio. b). Dose-response characteristic for the TNFα assay, showing enhanced signal using the PC surface. Full assay details are provided inAnal. Chem. Vol. 80, No. 23, p. 9013, 2008.

a). Fluorescent intensity images gathered with a confocal laser scanner for ~100 μm diameter spots of anti-TNFa capture probe after exposure to a 1 pg/ml solution of TNFα and subsequent labeling with Cy5-labeled secondary antibody. At this concentration, detection is near the detection limit when the assay is performed on a glass surface, but the PC surface shows robust signal-to-noise ratio. b). Dose-response characteristic for the TNFα assay, showing enhanced signal using the PC surface. Full assay details are provided inAnal. Chem. Vol. 80, No. 23, p. 9013, 2008.