Enhanced fluorescence a) and label-free b) images of 50 μg/ml SA-Cy5 spots on a PC. Inverted transmission versus angle response for a pixel inside and outside the SA-Cy5 spot c) and cross-section of the label-free image through 2 SA-Cy5 spots, in d). Rather than measuring the PWV, the label-free imaging system measures the Angle of Minimum Transmission (AMT) by illuminating the sensor at a fixed wavelength while scanning the angle of illumination through computer-controlled rotation of the mirror. (Reprinted with permission from the Optical Society of America. Optics Express, Vol. 17, No. 15, p. 13222,2009)