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. Author manuscript; available in PMC: 2010 Apr 6.
Published in final edited form as: Pediatr Blood Cancer. 2009 Aug;53(2):136–144. doi: 10.1002/pbc.21968

Fig. 5.

Fig. 5

Bortezomib restores Ptc1 and dampens Shh overdrive in human as well as mouse medulloblastoma cell lines. (A) in vitro cell growth assay of bortezomib in human medulloblastoma cell lines (DAOY, D283 and D341). IC50 ≤ 10nM. (B) Accumulation of Ptc1 protein (IP-Western) after treatment of bortezomib or transient transfection of Ptc1-YFP in both human (DAOY) and mouse (U26137) medulloblastoma cell lines. (C) Decrease of Gli1 transcription is accompanied with Ptc1 protein accumulation by either treatment of bortezomib (10 nM) or transfection of Ptc1-YFP in both human and mouse medulloblastoma cell lines. Ptc1 transcription is suppressed with treatment of bortezomib, while Ptc1-YFP transfected cell lines show redundant Ptc1 transcription.