Figure 4.

RIP-HGF transgenic islets display decreased FAO at high glucose concentrations. A, Oxidation of palmitate in RIP-HGF transgenic and normal (NL) mouse islets incubated with 3 and 16 mm glucose. Values are means ± sem of five different experiments in duplicate. *, P < 0.05 vs. 3 mm glucose; #, P < 0.05 vs. NL at 16 mm glucose. Representative images and densitometric quantitation of several Western blottings analyzing (B) AMPKα phosphorylation (Thr172), (C) ACC phosphorylation (Ser79), and (D) CPT-1 expression in protein extracts from RIP-HGF transgenic and NL mouse islets. The y-axis represents the ratio of phosphorylated vs. total or total vs. tubulin in arbitrary units. Values are means ± sem of four different islet extracts from RIP-HGF transgenic and NL mice. *, P < 0.05 vs. NL.