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DOC-PAGE analysis of the wild-type and mutant LPS. (a) LPS (1 μg) loaded in 1 μl sample buffer. (b) LPS extracted from 1 ml culture at OD600 0.5, as outlined in Methods. Lanes: S, standard LPS of Salmonella enterica serovar Minnesota (Sigma); 1, wild-type; 2, fabF2/F1−; 3, fabF2−; 4, fabF2/F1−pCS115; 5, fabF1−pCS115; 6, fabF1−. HMW, high-molecular-weight LPS; LMW, low-molecular-weight LPS.
